Molecular Characterisation and Proteomics

Towards the beginning of this century, mass spectrometry- based proteomics has emerged as a powerful tool for large scale screening, identification and quantitation of proteins from complex biological samples. Moving with this trend, a mass spectrometry (MS) facility was formed at NCBS with an aim to provide researchers state-of-the-art techniques to characterize biomolecules with an emphasis on proteins and peptides.

Services

 Currently, the following services are offered from this facility:                                                                                       

 1. Molecular mass measurement of intact proteins and peptides                                        PROTEIN ID SERVICES FOR ON-CAMPUS RESEARCHERS

 2. Identification of 1 D and 2 D gel separated proteins

 3. In-solution digestion and characterization of proteins                                                                           Click here to Submit

 4. De novo sequencing of peptides                                                                                                                        a request

 5. Quantitative Proteomics

• Isobaric Tag for Relative and Absolute Quantification (iTRAQ) labeling
• Stable Isotope Labeling (SILAC) (LC/MS only)

Facilities/Instruments

1) Agilent nano HPLC

This LC system with flow rate ranges from 100 nl/min to 1ul/min is suitable for high sensitivity MS detection and 2 dimensional separation.

2) Thermo LTQ Orbitrap Discovery

This MS with its mass resolution of 30,000 at 400 m/z and mass accuracy of 1-2 ppm is optimized for high throughput proteomics experiments. It has two fragmentation techniques, CID and PQD, associated with and is an excellent tool for labeled and label-free quantitation.

3) Waters Q-TOF Ultima

This sensitive instrument capable of performing MS and MS/MS experiments, is equipped with nano-electrospray source and on-line capillary HPLC, is mainly used for routine protein and peptide analysis.

4) Advion Triversa NanoMate Robot

Uses a chip based technology for stable sample infusion at nano litre flow rate.

MS Facility Service Flow Chart

1) Client writes to C-CAMP (details below) with service request

2) C-CAMP MS Facility management provides service requisition form to be filled, signed, scanned and sent back

3) Service requisition form is forwarded to MS Service team and assessed as to whether requested service can be provided and more information is requested from the client. If service is not able to be provided, client will be notified

4) If accepted, client is requested to send in their samples

5) Samples are processed and report/s are produced

6) Report/s along with invoice for services rendered are sent to client

Submitting a service request

To submit a request for MS services, please register by clicking here. After registration, please login and click on "My requests" in the menu bar followed by "New request".

The desired service form (General or Proteomics) can be filled and submitted to us.

Registered users can submit  a service request by clicking here.

Protocols & FAQs

In-gel digestion protocol                         nano LC-MS/MS protocol 

SDS-PAGE protocol                                 Protein/Peptide mass determination protocol 

Click here for clarifications to other commonly asked questions

Protein ID Services for on-campus researchers

1. Samples to be submitted at the Proteomics facility between 9am -12:30pm every Monday.

2. Facility Usage form approved and signed by PI should be submitted along with samples.

3. Data and report will be delivered by 6pm on Friday for the first 24 samples that are submitted by 12:30pm on Monday. In case of technical issues with instruments, we will not be able to deliver within specified time line.

4. This turn-around-time is limited to batches of 3 samples/ request. En-masse sample submissons (i.e. > 3 samples per request) will not be delivered within the above time frame.

For more information on proteomics, please write to services [at] ccamp.res.in or proteomics [at] ccamp.res.in

Technology Manager, Proteomics - Dr. Vikas Kumar

Business Development and Project Manager  - Dr. Mohammad Atif Alam

Ph: +91-80-67185052

Before availing our services, please read our Data Storage and Access Policy by clicking here