|ELYS Regulates Dorsal Dynamics during Development [Transgenic Fly Facility]
|Year of Publication
|Mehta SJayesh Kum, Kumar V, Mishra RKumar
|J Biol Chem
|2020 Jan 15
Embryonic large molecule derived from yolk sac (ELYS) is a constituent protein of nuclear pores. It initiates assembly of nuclear pore complexes (NPCs) into functional nuclear pores toward the end of mitosis. Using cellular, molecular and genetic tools, including fluorescence and electron microscopy, quantitative PCR and RNAi mediated depletion, here; we report that ELYS ortholog (Elys) plays critical roles during development. Elys localized to the nuclear rim in interphase cells, but during mitosis, it was absent from kinetochores and enveloped chromatin. We observed that RNAi-mediated depletion leads to aberrant development and, at the cellular level, to defects in the nuclear pore and nuclear lamina assembly. Further genetic analyses indicated that depletion re-activates the Dorsal (NF-κB) pathway during late larval stages. Re-activated Dorsal caused untimely expression of the Dorsal target genes in the post-embryonic stages. We also demonstrate that activated Dorsal triggers apoptosis during later developmental stages by up-regulating the pro-apoptotic genes and The apoptosis induced by Reaper and Hid was probably the underlying cause for developmental abnormalities observed upon depletion. Moreover, we noted that Elys has conserved structural features, but contains a non-canonical AT-hook like motif through which it strongly binds to DNA. Together, our results uncover a novel epistatic interaction that regulates Dorsal dynamics by Elys during development.
|J. Biol. Chem.