Analysis of smart biomaterial containing umbilical cord blood serum protein conjugated with P-(NIPAAM) using spectroscopy [Bio-incubation Services]

Human Umbilical Cord Blood Serum (HUCBS) is a complex and evolving collection of proteins that promote fetal development. In the realm of regenerative medicine, the important proteins found in HUCBS are of great interest. The smart biomaterial generated from HUCBS is described in this paper. To characterize this novel biomaterial, human umbilical cord blood was obtained in sterile vacutainers from mothers and left to clot for 24 h at 37 °C. The supernatant serum was collected, centrifuged and lyophilized. The lyophilized HUCBS was homogenized with smart polymer. This sample was subjected to physico-chemical characterization using Attenuated Total Reflectance-Fourier-Transform Infrared (ATR-FTIR) Spectroscopy and Nuclear Magnetic Resonance (NMR). The quantification of protein-polymer conjugate using ATR-FTIR revealed peaks ranging between 3264 and 531 cm−1 and that of NMR showed wide resonances in the region 0–5 ppm. ATR-FTIR and NMR investigations were used to determine the structural stability of protein molecules in protein-polymer complex which helps in understanding the possible clinical effectiveness of the smart biomaterial in drug delivery.