@article {1776,
	title = {Investigation of chemical and biological properties of an acidic polysaccharide fraction from Pleurotus eous (Berk.) Sacc},
	journal = {Food Bioscience},
	year = {2021},
	month = {06/2021},
	abstract = {<p>Pleurotus eous, pink oyster mushroom and functional food, is widely cultivated in Southern India. In this study, we successfully attempted, to isolate a new acidic polysaccharide fraction (PEPA-1a) possessing a molecular weight of 8.926 {\texttimes} 104 Da from the fruiting bodies of Pleurotus eous through ion-exchange and gel-filtration chromatographic techniques. A monosaccharide composition comprising glucose, galactose, rhamnose, mannose, and N-acetyl galactosamine were found to have the corresponding mole percentages of 20.25, 56.75, 3.06, 11.07, and 8.86, respectively. The antioxidant activity was assessed through seven in vitro tests, PEPA-1a showed significant antioxidant activity in a dose-dependent way, with EC50 values stretching from 1.08 to 4.91 mg/mL. Analyses of PEPA-1a towards in vitro anti-tumour showed high activity against HT29 (IC50 = 233.50 μg/mL) and PC3 cells (IC50 = 230.80 μg/mL). The anticoagulant activity was estimated through APTT, PT and TT tests which show effective anticoagulant activity. Also, the congo red analysis revealed the triple-helical structures of carbohydrates. The results indicate that consumption of P eous polysaccharide may be beneficial for health and will serve as an exceptionally accessible natural source for food and pharmaceutical industries.</p>
},
	keywords = {Anti-tumour activity, Anticoagulant activity, Antioxidant activity, Characterization, Pleurotus eous, Polysaccharides},
	doi = {doi.org/10.1016/j.fbio.2021.101209},
	url = {https://www.sciencedirect.com/science/article/abs/pii/S2212429221003345$\#$!},
	author = {Sasikala Gunasekaran and Sudha Govindan and Prasanna Ramani}
}
