@article {8897,
	title = {Embryonic spatiotemporal expression pattern of Folded gastrulation suggests roles in multiple morphogenetic events and regulation by AbdA [Transgenic Fly (Drosophila) Facility]},
	journal = {G3 (Bethesda)},
	volume = {14},
	year = {2024},
	month = {2024 May 07},
	abstract = {<p>In Drosophila, the signaling pathway activated by the ligand Folded gastrulation (Fog) is among the few known G protein-coupled receptor (GPCR) pathways to regulate cell shape change with a well-characterized role in gastrulation. However, an understanding of the spectrum of morphogenetic events regulated by Fog signaling is still lacking. Here, we present an analysis of the expression pattern and regulation of fog using a genome-engineered Fog::sfGFP line. We show that Fog expression is widespread and in tissues previously not associated with the signaling pathway including germ cells, trachea, and amnioserosa. In the central nervous system (CNS), we find that the ligand is expressed in multiple types of glia indicating a prominent role in the development of these cells. Consistent with this, we have identified 3 intronic enhancers whose expression in the CNS overlaps with Fog::sfGFP. Further, we show that enhancer-1, (fogintenh-1) located proximal to the coding exon is responsive to AbdA. Supporting this, we find that fog expression is downregulated in abdA mutants. Together, our study highlights the broad scope of Fog-GPCR signaling during embryogenesis and identifies Hox gene AbdA as a novel regulator of fog expression.</p>
},
	keywords = {Animals, Central Nervous System, Drosophila melanogaster, Drosophila Proteins, Embryo, Nonmammalian, Enhancer Elements, Genetic, Gastrulation, Gene Expression Regulation, Developmental, Morphogenesis, Signal Transduction, Transcription Factors},
	issn = {2160-1836},
	doi = {10.1093/g3journal/jkae032},
	author = {Katagade, Vrushali and Kandroo, Manisha and Ratnaparkhi, Anuradha}
}
@article {475,
	title = {Genetic transformation of structural and functional circuitry rewires the Drosophila brain. [Drosophila Facility]},
	journal = {Elife},
	volume = {3},
	year = {2014},
	month = {2014 Dec 29},
	abstract = {<p class="rtejustify">Acquisition of distinct neuronal identities during development is critical for the assembly of diverse functional neural circuits in the brain. In both vertebrates and invertebrates, intrinsic determinants are thought to act in neural progenitors to specify their identity and the identity of their neuronal progeny. However, the extent to which individual factors can contribute to this is poorly understood. We investigate the role of orthodenticle in the specification of an identified neuroblast (neuronal progenitor) lineage in the Drosophila brain. Loss of orthodenticle from this neuroblast affects molecular properties, neuroanatomical features, and functional inputs of progeny neurons, such that an entire central complex lineage transforms into a functional olfactory projection neuron lineage. This ability to change functional macrocircuitry of the brain through changes in gene expression in a single neuroblast reveals a surprising capacity for novel circuit formation in the brain and provides a paradigm for large-scale evolutionary modification of circuitry.</p>
},
	keywords = {Animals, Brain, Cell Lineage, Drosophila, Morphogenesis, Neurons},
	issn = {2050-084X},
	doi = {10.7554/eLife.04407},
	author = {Sen, Sonia and Cao, Deshou and Choudhary, Ramveer and Biagini, Silvia and Wang, Jing W and Reichert, Heinrich and VijayRaghavan, K}
}
