@article {533,
	title = {A rapid, nonradioactive assay for measuring heparan sulfate C-5 epimerase activity using hydrogen/deuterium exchange-mass spectrometry.},
	journal = {Methods Mol Biol},
	volume = {1229},
	year = {2015},
	month = {2015},
	pages = {209-19},
	abstract = {<p>Heparin and heparan sulfate (HS) glycosaminoglycans have important roles in anticoagulation, human development, and human diseases. HS C5-epimerase, which catalyzes the epimerization of GlcA to IdoA, is a crucial enzyme involved in the biosynthesis of heparin-related biomolecules. Here, we describe a detailed method for measuring the total activity of HS C5-epimerase that involves the following steps: H/D exchange upon epimerization of the substrate with HS C5-epimerase, low-pH nitrous acid treatment of the substrate, the separation of low-pH nitrous acid-cleaved disaccharides using HPLC, and mass spectrometry analysis. This nonradioactive method is rapid and sensitive and, importantly, allows us to study the reversible nature of HS C5-epimerase.</p>
},
	keywords = {Animals, Biocatalysis, Carbohydrate Epimerases, Chromatography, Ion Exchange, Chromatography, Liquid, Deuterium Exchange Measurement, Disaccharides, Enzyme Assays, Glucuronic Acid, Heparitin Sulfate, Humans, Iduronic Acid, Mass Spectrometry, Sf9 Cells},
	issn = {1940-6029},
	doi = {10.1007/978-1-4939-1714-3_19},
	author = {Babu, Ponnusamy and Victor, Xylophone V and Raman, Karthik and Kuberan, Balagurunathan}
}
@article {534,
	title = {Glycans in regeneration.},
	journal = {ACS Chem Biol},
	volume = {9},
	year = {2014},
	month = {2014 Jan 17},
	pages = {96-104},
	abstract = {<p>Glycans participate in many key cellular processes during development and in physiology and disease. In this review, the functional role of various glycans in the regeneration of neurons and body parts in adult metazoans is discussed. Understanding glycosylation may facilitate research in the field of stem cell biology and regenerative medicine.</p>
},
	keywords = {Animals, Extremities, Glycosylation, Humans, Hydra, Nerve Regeneration, Neurons, Polysaccharides, Regeneration, Regenerative Medicine},
	issn = {1554-8937},
	doi = {10.1021/cb400784j},
	author = {Babu, Ponnusamy}
}
