@article {2589,
	title = {Novel studies of characterization, antioxidant, anticoagulant and anticancer activity of purified polysaccharide from Hypsizygus ulmarius mushroom [Mass Spectrometry - Glycomics]},
	journal = {Bioactive Carbohydrates and Dietary Fibre},
	volume = {27},
	year = {2022},
	pages = {100308},
	abstract = {<p>A water-soluble polysaccharide (HUP-2) was isolated from H. ulmarius using hot water extraction and purified using ion exchange chromatography (DEAE cellulose-52) and gel filtration (Sepharose-6B). The structure characteristics of HUP-2 were investigated using high performance liquid chromatography (HPLC), Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectrum, and gas chromatography-mass spectrometry (GC-MS), and XRD analysis revealed that it formed a semi-crystalline structure. HUP-2 is a monosaccharide with a molecular weight of 25.296\&nbsp;kDa. The HUP-2 is assemble with 1.96\% rhamnose, 16.51\% mannose, 22.76\% galactose, and 19.43\% glucose. It could be found that the main backbone chain of HUP-2 consisted N-acetylglycosidic. The polysaccharide{\textquoteright}s DPPH radical scavenging activity ranged from 17.08 to 56.90\%. The radical scavenging activity of ABTS varied from 8.02 to 71.44\% at 0.5{\textendash}2.5\&nbsp;mg/ml, whereas superoxide radical scavenging activity was 8.02{\textendash}63.03\%. The results of HUP-2 demonstrated an extension (p\&nbsp;\&lt;\&nbsp;0.01) of APTT relative to the saline sample at concentrations more than 5\&nbsp;mg/mL. It exhibited a high antioxidant capacity as well. HUP-2 had significant inhibitory and cytotoxic effects in PC3 cells. HUP-2 arrested the cell cycle in the G0/G1 phase over all cells except PC3 cells. HUP-2 appears to have a wide range of biological applications, making it a good candidate for usage in functional foods, according to these studies.</p>
},
	keywords = {Anticancer, Anticoagulant, Antioxidant, HUP-2},
	issn = {2212-6198},
	doi = {https://doi.org/10.1016/j.bcdf.2022.100308},
	url = {https://www.sciencedirect.com/science/article/pii/S2212619822000031},
	author = {Alamelu Thimmaraju and Sudha Govindan}
}
@article {1776,
	title = {Investigation of chemical and biological properties of an acidic polysaccharide fraction from Pleurotus eous (Berk.) Sacc},
	journal = {Food Bioscience},
	year = {2021},
	month = {06/2021},
	abstract = {<p>Pleurotus eous, pink oyster mushroom and functional food, is widely cultivated in Southern India. In this study, we successfully attempted, to isolate a new acidic polysaccharide fraction (PEPA-1a) possessing a molecular weight of 8.926 {\texttimes} 104 Da from the fruiting bodies of Pleurotus eous through ion-exchange and gel-filtration chromatographic techniques. A monosaccharide composition comprising glucose, galactose, rhamnose, mannose, and N-acetyl galactosamine were found to have the corresponding mole percentages of 20.25, 56.75, 3.06, 11.07, and 8.86, respectively. The antioxidant activity was assessed through seven in vitro tests, PEPA-1a showed significant antioxidant activity in a dose-dependent way, with EC50 values stretching from 1.08 to 4.91 mg/mL. Analyses of PEPA-1a towards in vitro anti-tumour showed high activity against HT29 (IC50 = 233.50 μg/mL) and PC3 cells (IC50 = 230.80 μg/mL). The anticoagulant activity was estimated through APTT, PT and TT tests which show effective anticoagulant activity. Also, the congo red analysis revealed the triple-helical structures of carbohydrates. The results indicate that consumption of P eous polysaccharide may be beneficial for health and will serve as an exceptionally accessible natural source for food and pharmaceutical industries.</p>
},
	keywords = {Anti-tumour activity, Anticoagulant activity, Antioxidant activity, Characterization, Pleurotus eous, Polysaccharides},
	doi = {doi.org/10.1016/j.fbio.2021.101209},
	url = {https://www.sciencedirect.com/science/article/abs/pii/S2212429221003345$\#$!},
	author = {Sasikala Gunasekaran and Sudha Govindan and Prasanna Ramani}
}
