@article {2589,
	title = {Novel studies of characterization, antioxidant, anticoagulant and anticancer activity of purified polysaccharide from Hypsizygus ulmarius mushroom [Mass Spectrometry - Glycomics]},
	journal = {Bioactive Carbohydrates and Dietary Fibre},
	volume = {27},
	year = {2022},
	pages = {100308},
	abstract = {<p>A water-soluble polysaccharide (HUP-2) was isolated from H. ulmarius using hot water extraction and purified using ion exchange chromatography (DEAE cellulose-52) and gel filtration (Sepharose-6B). The structure characteristics of HUP-2 were investigated using high performance liquid chromatography (HPLC), Fourier transform infrared (FT-IR), nuclear magnetic resonance (NMR) spectrum, and gas chromatography-mass spectrometry (GC-MS), and XRD analysis revealed that it formed a semi-crystalline structure. HUP-2 is a monosaccharide with a molecular weight of 25.296\&nbsp;kDa. The HUP-2 is assemble with 1.96\% rhamnose, 16.51\% mannose, 22.76\% galactose, and 19.43\% glucose. It could be found that the main backbone chain of HUP-2 consisted N-acetylglycosidic. The polysaccharide{\textquoteright}s DPPH radical scavenging activity ranged from 17.08 to 56.90\%. The radical scavenging activity of ABTS varied from 8.02 to 71.44\% at 0.5{\textendash}2.5\&nbsp;mg/ml, whereas superoxide radical scavenging activity was 8.02{\textendash}63.03\%. The results of HUP-2 demonstrated an extension (p\&nbsp;\&lt;\&nbsp;0.01) of APTT relative to the saline sample at concentrations more than 5\&nbsp;mg/mL. It exhibited a high antioxidant capacity as well. HUP-2 had significant inhibitory and cytotoxic effects in PC3 cells. HUP-2 arrested the cell cycle in the G0/G1 phase over all cells except PC3 cells. HUP-2 appears to have a wide range of biological applications, making it a good candidate for usage in functional foods, according to these studies.</p>
},
	keywords = {Anticancer, Anticoagulant, Antioxidant, HUP-2},
	issn = {2212-6198},
	doi = {https://doi.org/10.1016/j.bcdf.2022.100308},
	url = {https://www.sciencedirect.com/science/article/pii/S2212619822000031},
	author = {Alamelu Thimmaraju and Sudha Govindan}
}
