Microfluidics and Microfabrication Facility

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Microfluidics and Microfabrication Facility

Microfluidics and microfabricated surface patterns are starting to be of growing importance in various disciplines of science, especially in biology. The possibility of addressing very small volumes and single cells is beginning to be leveraged to provide information, hitherto unavailable or unfeasible to be obtained by classical techniques, or even just to reduce the cost of the experiment. Towards this microstructures made of inert polymers like polydimethylsiloxane (PDMS), polycarbonate (PC), etc. are being used to  construct a varying array of fluidic circuits [1-5], designs of localized proteins [6-8], and 3-dimensional structures [9].

 

Objectives

The microfluidics and microfabrication facility at the Centre for Cellular and Molecular Platforms (C-CAMP) offers microfabrication service for the production of microfluidic devices, patterned substrates and stamped patterns utilising PDMS and to be extended to the other polymers. The facility is to be setup over the next few months with devices being produced utilising the Centre for Nano Science and Engineering at the Indian Institute of Science for specialised equipment not available at the M&M Facility.

 

Services offered

  • Microfluidic Devices
  • Micropatterned Substrates
  • Stamped Patterns
  • Technical expertise
  • Equipment access

We are currently in the process of designing and procuring the photomasks and molds from CeNSE. Please contact if you would like to club your experimental design in this order.

 

Contact Us

For more information on utilizing the Microfluidics and Microfabrication facility, please write to microfab[at]ccamp.res.in or services[at]ccamp.res.in  

Technology Manager,  Microfluidics and Microfabrication Facility - Feroz MH Musthafa

 

References:

1.  Fu, A.Y., et al., A microfabricated fluorescence-activated cell sorter. Nature biotechnology, 1999. 17(11): p. 1109-11.

2.  Lucchetta, E.M., et al., Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics. Nature, 2005. 434(7037): p. 1134-8.

3.  Sanchez-Freire, V., et al., Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nature protocols, 2012. 7(5): p. 829-38.

4.  Lee, W.C., et al., High-throughput cell cycle synchronization using inertial forces in spiral microchannels. Lab on a chip, 2011. 11(7): p. 1359-1367.

5.  Tan, S.J., et al., Versatile label free biochip for the detection of circulating tumor cells from peripheral blood in cancer patients. Biosensors & bioelectronics, 2010. 26(4): p. 1701-1705.

6.  Chen, C.S., et al., Micropatterned surfaces for control of cell shape, position, and function. Biotechnology progress, 1998. 14(3): p. 356-63.

7.  Kim, C.H., G.W. Kim, and H.J. Chun, Submicron-Patterned Fibronectin Controls the Biological Behavior of Human Dermal Fibroblasts. Journal of nanoscience and nanotechnology, 2010. 10(10): p. 6864-6868.

8.  Poellmann, M.J., et al., Patterned Hydrogel Substrates for Cell Culture with Electrohydrodynamic Jet Printing. Macromolecular bioscience, 2011. 11(9): p. 1164-1168.

9.  le Digabel, J., et al., Microfabricated substrates as a tool to study cell mechanotransduction. Medical & biological engineering & computing, 2010. 48(10): p. 965-76.