Mass Spectrometry-Lipidomics Facility

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The mass spectrometry (MS) resources on campus aim to provide researchers with state-of-the-art techniques and equipment to characterize biomolecules including proteins and peptides (proteomics, sugars including glycoproteins (glycomics), metabolites (metabolomics) and lipids (lipidomics). A number of state of the art instruments for the separation of biomolecules and their analysis by mass spectrometry based approaches are available. In addition to providing analytical services, the mass spectrometry facility on campus provides training to on-campus scientific staff on the use of this technology as well as develop new analytical methods required to facilitate on campus research.

Lipidomics:

Facility is driven to establish a hyphenated Mass Spectrometry based Lipidomics platform in basic biology, clinical studies and biotechnology at Bangalore Life Sciences Cluster (BLiSc) affiliated institutes; NCBS, CCAMP and InStem. The platform undertakes clinical, biological, chemical, ecology studies of lipidomics/ metabolites for BLiSc community and external researchers. Direct infusion as well as LC-MS/MS methods using High Resolution Mass Spectrometry (HRMS) like Orbitrap technology (Q-Exactive, Orbitrap XL) are available.

Identification and quantification of known and unknown Lipids/metabolites in biological fluids such as plasma, urine, saliva and tissue using Data dependant (DDA) and data independent (DIA) analysis for the estimation of lipids and other metabolites. Comprehensive lipid profiling and quantitation is a system-based study of all possible lipid classes in the biological system. Phosphoinositides measurements in cell signaling.

Lab is also well equipped with other sample preparation instruments such as solid phase extraction (SPE) and liquid-liquid extraction (LLE).

Well versed training program is available for the internal as well as external researchers throughout the year.